Alcohol dehydrogenase interference in lactate dehydrogenase assay.

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Evaluation of potential lactate/lactate dehydrogenase interference with an enzymatic alcohol analysis.

The Connecticut Department of Public Safety laboratory recently addressed a legal challenge to a hospital alcohol dehydrogenase (ADH)-based serum ethanol determination based on the suggestion of interference by lactate dehydrogenase (LDH)-catalyzed oxidation of lactate. Both ADH- and LDH-oxidations require NAD(+) (present in excess in the assay). NADH produced by LDH-catalyzed lactate oxidation...

متن کامل

Carbon nanodots interference with lactate dehydrogenase assay in human monocyte THP-1 cells

BACKGROUND Carbon nanodots (CD), a new class of carbon nanomaterials with sizes below 10 nm, have recently attracted wide attention due to their superiority in water solubility, chemical inertness, and resistance to photobleaching. As a result, CD has found important and wide applications in energy, catalysis, biological labeling, bioimaging and drug delivery. On the other hand, due to the lack...

متن کامل

Characterization of rabbit lactate dehydrogenase-M and lactate dehydrogenase-H cDNAs. Control of lactate dehydrogenase expression in rabbit muscle.

Two cDNA clones were isolated, one corresponding to the mRNA coding for lactate dehydrogenase-M (LDH-M), the other to the mRNA coding for lactate dehydrogenase-H (LDH-H). The cDNA inserts consist of the entire open reading frame for LDH-M and a partial sequence, from amino acid 117 to 332, for LDH-H. Using these two clones as probes we demonstrate that: (a) the abundance of mRNA is muscle-type ...

متن کامل

Serum Lactate Dehydrogenase Elevation

Activity of total lactate dehydrogenase (LDH) and that of the heat-stable isozyme (LDH-1) was measured by the method of Strandjord and Clayson in the serum of 62 cardiac patients with congenital and valvular heart disease before and after cardiac catheterization and at follow-up examination after cardiac surgery. A universal elevation of both total and heat-stable LDH was found in patients with...

متن کامل

Porcine Heart Lactate Dehydrogenase

The tinetics of NADH, 3-thionicotinamide adenine dinucleotide (TNAD), and oxamate binding to the H4 isoenzyme of lactate dehydrogenase from the pig has been investigated by temperature jump techniques. The dissociation rate constant for TNAD is considerably larger than for NADH, whereas the recombination rate constant is smaller for the oxidized coenzyme than for the reduced molecule. The kinet...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Clinical Chemistry

سال: 1980

ISSN: 0009-9147,1530-8561

DOI: 10.1093/clinchem/26.3.525